Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction.
نویسندگان
چکیده
Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair suggesting applications in genetic analysis.
منابع مشابه
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ورودعنوان ژورنال:
- Chemical communications
دوره 47 22 شماره
صفحات -
تاریخ انتشار 2011